Formula 99C: Cold-Temperature Bacteria Supplement for Wastewater

Low temperatures in wastewater can create challenges, such as reduced biological activity and increased solidification of fats, oils, and grease.

Formula 99C is a specialized blend of psychrophilic bacteria that thrive in colder water, effectively producing enzymes and metabolizing BOD. It maintains consistent treatment performance even when wastewater temperatures drop as low as 36ºF.

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Formula 99C Features


  • Industrial-strength dry bacteria formulation

  • Biodegradable

  • Contains special micronutrients and stimulants for rapid integration into existing biomass

  • Packaged in convenient, ready-to-use 1/2-lb water-soluble bags

  • Thrives in environments without "free" or dissolved oxygen

  • Environmentally friendly

  • Available in 25-lb pail (Product# 146925)

Formula 99C Benefits


  • Optimizes system performance in low temperatures by enhancing microbial growth and activity

  • Degrades various wastes, including surfactants, solvents, polymer residues, starch, cellulose, FOG, and petroleum hydrocarbons

  • Reduces hydrogen sulfide

  • Enhances BOD, COD, and TSS removal

  • Contains a unique blend of psychrophilic (cold-loving) bacteria to sustain biological activity in water temperatures below 50°F

  • Efficiently produces enzymes and metabolizes BOD at temperatures as low as 36°F

Using Formula 99C


Dosing Schedule:
Flow Rate Initial Dosage Maintenance
Up to 1,000 GPD ½ lb. per day for 3 days ½ lb. per week
Up to 5,000 GPD ½ lb. per day for 3 days 1 lb. per week
Up to 20,000 GPD 5 lbs.* 1 ½ lb. per week
Up to 50,000 GPD 8 lbs.* 2 lb. per week
Up to 250,000 GPD 15 lbs.* ¼lb. per day
Up to 500,00 GPD 25 lbs.* ½lb. per day
Up to 1 MGD 50 lbs.* 1 lb. per day
Up to 5 MGD 50 lbs. per MGD* 1 lb. per MGD per day
Up to 12 MGD 50 lbs. per MGD* ¾ lb. per MGD per day
Up to 100 MGDD 30 lbs. per MGD* ½ lb. per MGD per day
* Spread this initial dosage out over the course of 10 days.

* * Add as regularly as possible. If it is required to miss one day, add that day’s product with the next dosage.

Dosage rate will vary with flow rates, retention times and system variations.

Do not add to the treatment system at a location where toxic or otherwise adverse pH, dissolved oxygen or temperature conditions may exist at peak levels.

For optimal results the wastewater and treatment system should meet the following conditions:
Optimum Minimum
Influent pH 7.0 5.0
Dissolved oxygen, ppm 2.0+ 1.0+
C/N/P ratio 100/10/1 100/5/1
Temperature 30°C (86°F) 10°C (50°F)
Toxic metals, ppm
(E.g., hex., chromium)		 0 0

Storage and Handling:

Storage: Store in a cool, dry place.

Handling: Wash hands thoroughly with warm, soapy water after handling.

See label and SDS for safety information.


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